lactococcus lactis pathogenicity

2014 Nov;304(8):1233-46. doi: 10.1016/j.ijmm.2014.10.001. Epub 2014 Oct 14. Twenty-eight Lactococcus isolates were obtained from mastitic milk of 28 dairy cows. Clin Vaccine Immunol. After intranasal challenge with M28 streptococci, survival of M×10-immunized mice was significantly higher than that of unimmunized mice. To assess the exclusive impact of pneumococcal surface protein C (PspC) on the interplay with its host we generated recombinant L. lactis producing a nisin-inducible and covalently anchored variant of PspC on the lactococcal cell surface. L. lactis subsp. Getting to the heart of the matter: Role of Streptococcus mutans adhesin Cnm in systemic disease. Epub 2018 Jun 11. For example, certain variants of Lactococcus species have been associated with bovine mastitis (e.g., Lactococcus lactis and Lactococcus garvieae). Epub 2016 Jul 13. However, this N- terminal fragment is hypervariable: there are more than 200 different M types. Lactococcus lactis is a non-pathogenic bacterium that is used in the food industry but is also used as a heterologous host to reveal protein functions of pathogenic bacteria. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Curr Opin Infect Dis. 2005 Oct;68(6):705-17. doi: 10.1007/s00253-005-0107-6. Binsker U, Kohler TP, Krauel K, Kohler S, Schwertz H, Hammerschmidt S. J Biol Chem. The host immune regulator factor H interacts via two contact sites with the PspC protein of Streptococcus pneumoniae and mediates adhesion to host epithelial cells. Furthermore, L. lactis expressing PspC increased intracellular calcium levels in pIgR-expressing epithelial cells, thus resembling the effect of pneumococci, which induced release of Ca(2+) from intracellular stores via the PspC-pIgR mechanism. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: See this image and copyright information in PMC. This site needs JavaScript to work properly. Efficacy evaluation of vaccine strains tested individually, Figure 5. COVID-19 is an emerging, rapidly evolving situation. 2011. Lactococcus lactis has been studied extensively until now as a technological bacterium used to produce fermented foods. Mx-10 immunized mice had significantly less S. pyogenes in oropharyngeal washes and developed less severe disease symptoms after challenge than did unimmunized mice. However, studies have also linked Lactococcus bacteria to infection. Protective immunogenicity of Mx10 vaccine, NLM 2004 Feb;3(1):43-58. doi: 10.1586/14760584.3.1.43. NLM 2007 May 1;178(9):5848-58. doi: 10.4049/jimmunol.178.9.5848. Efficacy evaluation of vaccine strains…, Figure 3. lactis (Lister 1873) Schleifer et al. This site needs JavaScript to work properly. All 10 strains combined in a 10-valent vaccine (M×10) induced serum and bronchoalveolar lavage IgG titers that ranged from three- to 10-fold those of unimmunized mice. This study shows also for the first time that PspC plays no significant role during phagocytosis by macrophages. Please enable it to take advantage of the complete set of features! García PC, Paillavil BA, Scioscia N, Dale JB, Legarraga P, Salazar-Echegarai FJ, Bueno SM, Kalergis AM, Wozniak A. Microbiol Immunol. Further studies should be conducted to understand the importance of Lactococcus, especially L. lactis, as pathogenic microbes in veterinary medicine and food safety. Expert Rev Vaccines. Asmat TM, Agarwal V, Saleh M, Hammerschmidt S. Int J Med Microbiol.  |  Live bacterial-vectored vaccines cost less to manufacture because the processes involved are less complex than those required for production of protein subunit vaccines. HHS Epub 2005 Oct 13. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Microbiome analysis determined Lactococcus to be the dominant genus in the majority of the mastitic milk samples, whereas it was found in low relative abundance in healthy milk samples. Microbiol Immunol. A translational fusion of the 5'-end of pspC3.4 with the 3'-end of hic (pspC11.4) was designed to decorate the surface of L. lactis with a chimeric PspC. Emerg Microbes Infect. Figure 1. 2020 Jun;33(3):244-250. doi: 10.1097/QCO.0000000000000644. 2011 May 20;286(20):17861-9. doi: 10.1074/jbc.M110.212225. Lactococcus lactis subsp. 2018 Jun;62(6):395-404. doi: 10.1111/1348-0421.12595. SDS-PAGE analysis of heterologous M…, Figure 1. R01 AI010085/AI/NIAID NIH HHS/United States, R01 AI132117/AI/NIAID NIH HHS/United States. Clinical and microbiological response of mice to intranasal inoculation with Lactococcus lactis expressing Group A Streptococcus antigens, to be used as an anti-streptococcal vaccine. 2014 Sep;21(9):1343-9. doi: 10.1128/CVI.00330-14. © 2018 The Societies and John Wiley & Sons Australia, Ltd. Our L. lactis-based vaccine may provide an alternative solution to development of broadly protective group A streptococcal vaccines. Keywords: Immunogenicity of vaccine strains evaluated individually, Figure 3.  |  2015;14(11):1459-70. doi: 10.1586/14760584.2015.1081817. Comparative genome-based identification of a cell wall-anchored protein from Lactobacillus plantarum increases adhesion of Lactococcus lactis to human epithelial cells. By continuing you agree to the use of cookies. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Open Archive in partnership with American Dairy Science Association (ADSA). Old synonyms: Streptococcus lactis (Lister 1873) Lohnis 1909, Streptococcus lactis subsp.  |  lactis (Lister 1873) Garvie and Farrow 1982, Bacterium lactis Lister 1873. 1986. Update on group A streptococcal vaccine development. The principal role of lactococci in dairy fermentations involves the metabolism of lactose to lactic acid and the conversion of … Moreover, intranasal administration does not require syringes or specialized personnel. Streptococcus pyogenes (group A Streptococcus) causes diseases ranging from mild pharyngitis to severe invasive infections. Epub 2014 Jul 23. Endocytosis of Streptococcus pneumoniae via the polymeric immunoglobulin receptor of epithelial cells relies on clathrin and caveolin dependent mechanisms. SDS-PAGE analysis of heterologous M protein expression, Figure 2. 2018 Mar 13;9(2):e00264-18. The authors declare No Conflict of Interest. Phylogenetic analysis based on 16S rDNA gene sequence comparison indicated similarity among the L. lactis isolates as well as between the isolates and reference sequences. Moreover, invasion via hpIgR was even more pronounced when the chimeric PspC was produced by lactococci compared with pneumococci. Lipid core peptide technology and group A streptococcal vaccine delivery. doi: 10.1038/emi.2014.23. Appl Microbiol Biotechnol. Wozniak A, García P, Geoffroy EA, Aguirre DB, González SA, Sarno VA, Dale JB, Salazar-Echegarai FJ, Vera A, Bueno SM, Kalergis AM. 4. A novel live vector group A streptococcal emm type 9 vaccine delivered intranasally protects mice against challenge infection with emm type 9 group A streptococci. Immunogenicity of vaccine strains evaluated…, Figure 2. Epub 2011 Mar 29. Infect Immun. 2010 Jul 1;77(1):1-5. doi: 10.1111/j.1365-2958.2010.07190.x. Please enable it to take advantage of the complete set of features!  |  In this study, an intranasal live bacterial vaccine comprising 10 strains of Lactococcus lactis, each expressing one N-terminal fragment of M protein, has been developed. For example, certain variants of Lactococcus species have been associated with bovine mastitis (e.g., Lactococcus lactis and Lactococcus garvieae). Clipboard, Search History, and several other advanced features are temporarily unavailable. We used bacterial isolation, next-generation sequencing, DNA fingerprinting, and other methods to test our hypothesis that Lactococcus microbes were the primary pathogen causing the mastitis outbreak. 1. This is a common starter used to make soft cheeses, Cheddar-type cheeses, and many fermented milks throughout the world. In contrast, recruitment of Factor H via the PspC chimer has a dramatic effect on phagocytosis of recombinant but not wild-type lactococci, as Factor H interacts specifically with the amino-terminal part of PspC and mediates the contact with phagocytes. 2015 Jun 5;290(23):14542-55. doi: 10.1074/jbc.M114.623876. The adhesin PspC from Streptococcus pneumoniae is a choline-binding protein that is non-covalently anchored to the bacterial cell wall. Get the latest public health information from CDC: 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis.  |  Epub 2015 Apr 20. Epub 2010 Apr 27. Group A Streptococcus; M protein; intranasal vaccine; live bacterial vaccine. The N-terminal fragment of streptococcal M protein elicits protective antibodies and is an attractive vaccine target. Streptococcus pneumoniae infection of host epithelial cells via polymeric immunoglobulin receptor transiently induces calcium release from intracellular stores. 2018 Nov;62(11):711-719. doi: 10.1111/1348-0421.12657. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: Mucosal vaccine made from live, recombinant Lactococcus lactis protects mice against pharyngeal infection with Streptococcus pyogenes. The Lactococcus genus was detected in all environmental samples tested, and sampling of bulk tank milk corroborated that Lactococcus was not abundant in healthy milk from the same dairy herd. Get the latest research from NIH: Copyright © 2020 Elsevier B.V. or its licensors or contributors. Clipboard, Search History, and several other advanced features are temporarily unavailable. The DNA fingerprinting analysis based on random amplified polymorphic DNA results of the 27 L. lactis isolates identified different random amplified polymorphic DNA profiles, which suggests they originated from multiple sources.

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