weissella confusa fermentation

A further important feature for potential microbial starter strains is the ability to acidify its environment. Kostinek et al. Virkki nov., detected in food and clinical samples. These text files were imported into the BioNumerics 6.0 software package (Applied- Maths, Sint-Martens-Latem, Belgium) and converted to fingerprints for further analysis. Van Geel-Schutten Viscosity Measurement of Fermented Milk Glucan concentration in the standard medium was 34 mg/ml which on addition of glucose (3%) significantly increased 3 fold to 100 mg/ml (Fig. In our earlier study high glucan production ability of Weissella confusa (Cab3) isolated from fermented cabbage was explored and compared with other lactic acid bacteria (Shukla and Goyal, 2011). The unique band detected at 180 kDa was excised from the Coomassie blue-stained gel in sterile conditions and stored in ultrapure water at 4 °C. M [ Links ], Fox, J.D. These two species could be distinguished by their ability to produce acid from arabinose, in agreement with Björkroth & Holzapfel (2006). Taxonomic study of Weissella confusa and description of Weissella cibaria sp. MG Kalantzopoulos (1997), Dextransucrase production by Leuconostoc mesenteroides NRRL B-1299. The sourdough strain K39, which produced the highest soluble enzyme activity, was selected to perform protein sequencing in order to design specific primers. Food Microbiol., 75(3), 197-212. RM None of the strains synthesized β-glucosidase. S (Doan et al., 2012). Carbohydr. lactis and was stopped when the pH reached the value of 4.5 by rapid cooling of fermented milk up to 4°C. The medium containing the 2% sucrose and 0% glucose was considered as control. Faucher 107: 104414. These two species have been isolated from a wide variety of fermented products of plant origin (Björkroth et al., 2002; Camu et al., 2007; Kostinek et al., 2007; Chao et al., 2008), in particular from sourdough (De Vuyst et al., 2002; Catzeddu et al., 2006; Valmorri et al., 2006; Iacumin et al., 2009; Robert et al., 2009). Nat. Environ., 17(4), 634-643. (1979), Production, purification, and properties of dextransucrase from Leuconostoc mesenteroides NRRL B-512F. As the concentration increased beyond 2%, there was no change in enzyme activity. Juvonen JF Increment in sucrose concentration is also known to enhance the enzyme activity in other lactic acid strains viz. D Evol. Food Microbiol., 26, 734-743. A a Pearson correlation of 99.5% for isolates Wc 21 and Wc 114 and 99.7% for Wc 69 (Figure 1). P Virkki Glucan synthesis is catalyzed from sucrose by secreted or cell-anchored glucansucrases, which convert the sucrose substrate into high-molecular-weight polymers, with the concomitant release of fructose (Monsan et al., 2001; van Hijum et al., 2006). Briefly, after strain precultivation in MRS broth at 25 °C, a 100 mL culture was prepared (initial OD550 nm=0.3) in plain MRS (glucose medium) or in MRS containing 4% w/v sucrose instead of 2% w/v glucose (sucrose medium). Smit Key words: Weissella confusa, glucansucrase, glucan, Tween 80. The presence of coliform was mentioned in traditional cassava starters and in the cassava dough during attiéké production. M Also, from gari fermentation process, the potential microbial starters induced a drop of the MRS broth pH to less than 3.9 after 48 h growth (Kostinek et al., 2007). The dextransucrase production level of the different Weissella strains cultivated with sucrose or glucose as the carbon source was determined and compared with those obtained from the well-characterized dextran-producing strain L. mesenteroides NRRL B-512F (Fig. Weissella total DNA was prepared according to Robert (2009) or using a DNA extraction kit (DNeasy Blood and Tissue kit, Qiagen) from overnight cultures grown in MRS medium. (, Chao 8The three isolates synthesized α-amylase and cell wall-degrading enzyme pectinase for isolate Wc 114, and they fermented raffinose which is an indigestible sugar. Conversely, no active band was detected with the reference strain NRRL B-512F when cultivated in glucose growth conditions (Fig. Indeed, spoilage bacteria, as well as pathogens, notably those including members of Enterobacteriaceae, do not grow below this pH (Kostinek et al., 2007). PCR amplifications were carried out using a Gradient Master Thermocycler (Eppendorf). V It is worth recalling here that the acid production and the accompanying pH decrease are well-known to extend the lag phase of sensitive organisms including foodborne pathogens. Addition of glucose in the medium also significantly increased the glucan production. Willemot [ Links ], Funane, K., Yamada, M. Shiraiwa, M. Takahara, H. Yamamoto, N. Ichishima, E. and Kobayashi, M. (1995), Aggregated form of dextransucrases from Leuconostoc mesenteroides NRRL B-512F and its constitutive mutant.

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